Hayward Cultured in Vitro
نویسندگان
چکیده
An efficient procedure for shoot regeneration was obtained by endosperm culture in Actinidia deliciosa cv. Hayward. Mature endosperm cultured on MS medium supplemented with 2 mg/l 2,4-D and 5 mg/l kinetin developed callus with 80% efficiency. Callus was transferred on MS medium containing different plant growth regulators (2,4-D, TDZ, IAA, NAA, BAP, kinetin, 2iP) for regeneration. There were significant differences in regeneration response between medium supplemented with TDZ and medium with other hormones. Only medium containing TDZ stimulated shoot induction. The highest efficiency of shoot regeneration (avg. 6.2 shoots per culture) was on medium supplemented with 0.5 mg/l TDZ. The ploidy of callus and organs formed in endosperm culture was examined by flow cytometry. The results, peaks corresponding to 3C DNA amounts, confirmed the endospermal origin of callus, roots and shoots. Aneuploid and polyploid cells were found in endosperm-derived callus and regenerated organs.
منابع مشابه
اثر بستهبندی و انبارمانی بر خصوصیات فیزیکوشیمیایی دو رقم کیوی
The Two cultivars of kiwi fruit, Hayward and Abbot, with the desirable quality were harvested on time from Valiabad, a region in Tonekabon (Mazanddaran Provicnce). Fruits were graded and packed in the wooden boxes, cartons and low density polyethylene (LDPE) films, the fruits were cold stored in a proper and constant conditions (T= ±0.5 & RH=90-95%) for a period of 6 months. Samples were taken ...
متن کاملSildenafil Effect on Nitric Oxide Secretion by Normal Human Endometrial Epithelial Cells Cultured In vitro
متن کامل
Role of Follicle Stimulating Hormone in the Survival, Activation and Further Growth of in vitro Cultured Sheep Primordial Follicles
The aim of the present study was to investigate the effect of follicle stimulating hormone (FSH) on survival, activation and growth of ovine primordial follicles using histological studies. Pieces of ovine ovarian cortex were obtained and cultured for 6 days in Minimum Essential Medium supplemented with or without FSH (50 ng/mL). These fragments were then process to be used for histology compar...
متن کاملEffect of Different Cold Stratification Periods on Breakdown of Hayward Kiwifruit Seed Dormancy
Kiwifruit is a fast-growing deciduous woody vine that can be propagated in several ways. Nevertheless, seedlings have a strong and widespread system of roots than cuttings. The aim of this study was to find a simple and practical method for farmers to increase the germination percentage of kiwifruit seeds. This study was performed in a completely randomized design with five treatments of Cold S...
متن کاملIdentification of excretory-secretory products from larval stages of Ostertagia circumcincta cultured in vitro by SDS-PAGE and immunoblotting
Exsheathing fluid (EF) and excretory-secretory products (ES) of infective third-stage cultured larvae ofOstertagia circumcincta were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDSPAGE). Five and seven predominant proteins were found in the EF and ES products, respectively. Immunoblotting by sheep pre-infection serum did not react with any of the EF and ES proteins, b...
متن کامل